Synthetic Biology and Engineering

Open Access  ISSN: 2958-9053 (Online);  2958-9045 (Print)

Synthetic Biology and Engineering (SBE) is an international, peer-reviewed, open access journal dealing with interdisciplinary research of synthetic biology, from living systems to industry translation, published quarterly online by SCIEPublish.

Editor-in-Chief

An-Ping  Zeng Prof. Dr. An-Ping Zeng
Center of Synthetic Biology and Integrated Bioengineering, School of Engineering, Westlake University, Hangzhou, 310024, China
Shang-Tian  Yang Prof. Dr. Shang-Tian Yang
Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH 43210, USA

Editorial Board

Review Open Access
One-pot Multi-enzyme Cascade Synthesis of Bifunctional Compounds from Vegetable Oils
  • Synthetic Biology and Engineering 2024, 2(1), 10004; https://doi.org/10.35534/sbe.2024.10004
  • Xiaoxia Gao   Ran Lu   Yueyue Zhou   Lin Lu   Xiaojun Ji   Check
     21 Feb 2024

    Green and efficient biocatalytic technology has become a complementary or alternative means of organic synthesis. Chemicals with two functional groups...

    Review Open Access
    Development and Perspective of Production of Terpenoids in Yeast
  • Synthetic Biology and Engineering 2024, 2(1), 10003; https://doi.org/10.35534/sbe.2024.10003
  • Yaying Xia   Congna Li   Ruidi Cao   Lu Qin   Shuobo Shi   Check
     13 Feb 2024

    Terpenoids are a large class of secondary metabolites known for their remarkable diverse biological activities, making them widely utilized in the p...

    Article Open Access
    Bio-Based Production of Uroporphyrin in Escherichia coli
  • Synthetic Biology and Engineering 2024, 2(1), 10002; https://doi.org/10.35534/sbe.2024.10002
  • Bahareh Arab   Adam W. Westbrook   Murray Moo-Young   Yilan Liu   C. Perry Chou   Check
     06 Feb 2024

    Uroporphyrin (UP) is a porphyrin compound with medical applications and a key precursor for heme biosynthesis. However, there is no biosynthetic str...

    Article Open Access
    Analysis of a σ54 Transcription Factor L420P Mutation in Context of Increased Organic Nitrogen Tolerance of Photofermentative Hydrogen Production in Cereibacter sphaeroides Strain 2.4.1 Substrain H2
  • Synthetic Biology and Engineering 2024, 2(1), 10001; https://doi.org/10.35534/sbe.2024.10001
  • Nadine Wappler   Röbbe Wünschiers   Check
     23 Jan 2024

    Photofermentative hydrogen production with non-sulfur purple bacteria like Cereibacter sphaeroides (formerly Rhodobacter sphaeroides...

    Review Open Access
    Deciphering the Code of Pattern Formation: Integrating In Silico and Wet Lab Approaches in Synthetic Biology
  • Synthetic Biology and Engineering 2023, 1(3), 10018; https://doi.org/10.35534/sbe.2023.10018
  • Anqi Xu   Lizhong Liu   Jian-Dong Huang   Check
     27 Dec 2023

    Pattern formation is a fundamental process in biological development, enabling the transformation of initially uniform or random states into spatially...

    Article Open Access
    Serine Integrase-based Recombination Enables Direct Plasmid Assembly In Vivo
  • Synthetic Biology and Engineering 2023, 1(3), 10017; https://doi.org/10.35534/sbe.2023.10017
  • Luyao Wang   Yufei Zhang   Wan-Qiu Liu   Fang Ba   Jian Li   Check
     20 Dec 2023

    Serine integrases are emerging as one of the powerful tools for synthetic biology. They have been widely developed across genome engineering, biologic...

    Article Open Access
    Time-efficient and Semi-automated Production and Screening of Proteins
  • Synthetic Biology and Engineering 2023, 1(3), 10016; https://doi.org/10.35534/sbe.2023.10016
  • S. R. Sekar   S. Ilhan   Uwe Jandt   An-Ping Zeng   Check
     18 Dec 2023

    Fast, flexible and non-randomized modification, production and screening of proteins in fully automated system are of high interest in biological rese...

    Review Open Access
    Metabolic Engineering of Microorganisms Towards the Biomanufacturing of Non-Natural C5 and C6 Chemicals
  • Synthetic Biology and Engineering 2023, 1(3), 10015; https://doi.org/10.35534/sbe.2023.10015
  • Ashley Tseng   Vanna Nguyen   Yuheng Lin   Check
     31 Oct 2023

    Five-carbon (C5) and six-carbon (C6) chemicals are essential components in the manufacturing of a variety of pharmaceuticals, fuels, polymers, and oth...

    Commentary Open Access
    Synthetic Biology Industry in China: Current State and Future Prospects
  • Synthetic Biology and Engineering 2023, 1(2), 10014; https://doi.org/10.35534/sbe.2023.10014
  • Wei Luo   Yang Zhang   Jun Peng   Lishan Zhao   Check
     14 Sep 2023

    In this article, we provided an overview of the current state of the SynBio industry in China with a focus on its research and technology, its main ap...

    Review Open Access
    In Vitro BioTransformation (ivBT): Definitions, Opportunities, and Challenges
  • Synthetic Biology and Engineering 2023, 1(2), 10013; https://doi.org/10.35534/sbe.2023.10013
  • Yi-Heng P. Job Zhang   Zhiguang Zhu   Chun You   Lingling Zhang   Kuanqing Liu   Check
     01 Sep 2023

    Great needs always motivate the birth and development of new disciplines and tools. Here we propose in vitro BioTransformation (ivBT) as a ne...

    Review Open Access
    Dynamic Metabolic Control: From the Perspective of Regulation Logic
  • Synthetic Biology and Engineering 2023, 1(2), 10012; https://doi.org/10.35534/sbe.2023.10012
  • Tian Jiang   Chenyi Li   Yuxi Teng   Jianli Zhang   Diana Alexis Logan   Yajun Yan   Check
     28 Aug 2023

    Establishing microbial cell factories has become a sustainable and increasingly promising approach for the synthesis of valuable chemicals. However, i...

    Review Open Access
    Challenging Post-translational Modifications in the Cell-free Protein Synthesis System
  • Synthetic Biology and Engineering 2023, 1(2), 10011; https://doi.org/10.35534/sbe.2023.10011
  • Kassidy B. Porche   Claire E. Lanclos   Yong-Chan Kwon   Check
     28 Jul 2023

    Post-translational modifications (PTMs) represent a cornerstone in the complexity of the proteome, significantly contributing to diversifying protein ...

    Article Open Access
    Hydroxybenzoic Acid Production Using Metabolically Engineered Corynebacterium glutamicum
  • Synthetic Biology and Engineering 2023, 1(2), 10010; https://doi.org/10.35534/sbe.2023.10010
  • Misa Doke   Mayumi Kishida   Yuuki Hirata   Mariko Nakano   Mayo Horita   Daisuke Nonaka   Yutaro Mori   Ryosuke Fujiwara   Akihiko Kondo   Shuhei Noda   Tsutomu Tanaka   Check
     19 Jul 2023

    Hydroxybenzoic acids (HBAs), including 4-HBA, 3-HBA, and 2-HBA, are valuable platform chemicals for production of commodity materials and fine chemica...

    Article Open Access
    Nitrogen-controlled Valorization of Xylose-derived Compounds by Metabolically Engineered Corynebacterium glutamicum
  • Synthetic Biology and Engineering 2023, 1(2), 10009; https://doi.org/10.35534/sbe.2023.10009
  • Lynn Schwardmann    Marielle Rieks   Volker F. Wendisch   Check
     31 May 2023

    The implementation of bioprocesses in an economically feasible and industrial competitive manner requires the optimal allocation of resources for a ba...

    Article Open Access
    Fed-batch Self-regulated Fermentation of Glucose to Co-produce Glycerol and 1,3-propanediol by Recombinant Escherichia coli
  • Synthetic Biology and Engineering 2023, 1(2), 10008; https://doi.org/10.35534/sbe.2023.10008
  • Guimin Liu   Cai Feng   Zhiwei Zhu   Yaqin Sun   Zhilong Xiu   Check
     22 May 2023

    As important bio-chemicals, glycerol and 1,3-propanediol (1,3-PDO) have been widely used in numerous fields, e.g., polymers, cosmetics, foods, lubrica...

    Article Open Access
    Development of a New 1,2,4-butanetriol Biosynthesis Pathway in an Engineered Homoserine-producing Strain of Escherichia coli
  • Synthetic Biology and Engineering 2023, 1(1), 10007; https://doi.org/10.35534/sbe.2023.10007
  • Yujun Zhang   Lin Chen   Antu Thomas   An-Ping Zeng   Check
     10 May 2023

    1,2,4-butanetriol (BT) is a compound of high interest with applications in pharmaceutical and materials. In this work, we designed a novel biosynthe...

    Review Open Access
    Coiled Coils as Versatile Modules for Mammalian Cell Regulation
  • Synthetic Biology and Engineering 2023, 1(1), 10006; https://doi.org/10.35534/sbe.2023.10006
  • Estera Merljak   Anja Golob-Urbanc    Tjaša Plaper   Roman Jerala   Check
     06 Apr 2023

    Synthetic biology is a rapidly growing field that allows us to better understand biological processes at the molecular level, and enables therapeutic ...

    Review Open Access
    Thermoanaerobacter Species: The Promising Candidates for Lignocellulosic Biofuel Production
  • Synthetic Biology and Engineering 2023, 1(1), 10005; https://doi.org/10.35534/sbe.2023.10005
  • Kaiqun Dai   Chunyun Qu   Hongxin Fu   Jufang Wang   Check
     15 Mar 2023

    Thermoanaerobacter species, which have broad substrate range and high operating temperature, can directly utilize lig...

    Article Open Access
    Design of Oscillatory Networks through Post-translational Control of Network Components
  • Synthetic Biology and Engineering 2023, 1(1), 10004; https://doi.org/10.35534/sbe.2023.10004
  • Brianna E.K. Jayanthi   Shridhar Jayanthi   Laura Segatori   Check
     13 Mar 2023

    Many essential functions in biological systems, including cell cycle progression and circadian rhythm regulation, are governed by the periodic behav...

    Article Open Access
    Design of Oscillatory Networks through Post-translational Control of Network Components
  • Synthetic Biology and Engineering 2023, 1(1), 10004; https://doi.org/10.35534/sbe.2023.10004
  • Brianna E.K. Jayanthi   Shridhar Jayanthi   Laura Segatori   Check
     13 Mar 2023

    Many essential functions in biological systems, including cell cycle progression and circadian rhythm regulation, are governed by the periodic behav...

    Review Open Access
    Increasing Nutritional Value of Cyanobacteria by Engineering Valine, Phenylalanine, and Fatty Acid Production
  • Synthetic Biology and Engineering 2023, 1(1), 10003; https://doi.org/10.35534/sbe.2023.10003
  • Nick Lopez-Riveira   Stephen Fong   Check
     16 Feb 2023

    In 2020, the United Nations estimated that 2.37 billion people globally were without food or unable to eat a healthy balanced diet. The number of peop...

    Article Open Access
    Production of Highly Modified C30-carotenoids with Singlet Oxygen-quenching Activities, 5-glucosyl-5,6-dihydro-4,4’-diapolycopen-4’-oic Acid, and Its Three Intermediates Using Genes from Planococcus maritimus Strain iso-3
  • Synthetic Biology and Engineering 2023, 1(1), 10002; https://doi.org/10.35534/sbe.2023.10002
  • Moe Hagiwara   Chinatsu Maehara   Miho Takemura   Norihiko Misawa   Kazutoshi Shindo   Check
     07 Feb 2023

    Planococcus maritimus strain iso-3 was previously isolated from intertidal sediment in the North Sea and was found to produce a highly modifie...

    Review Open Access
    Thermoanaerobacter Species: The Promising Candidates for Lignocellulosic Biofuel Production
  • Synthetic Biology and Engineering 2023, 1(1), 10005; https://doi.org/10.35534/sbe.2023.10005
  • Kaiqun Dai   Chunyun Qu   Hongxin Fu   Jufang Wang   Check
     15 Mar 2023

    Thermoanaerobacter species, which have broad substrate range and high operating temperature, can directly utilize lig...

    Article Open Access
    Nitrogen-controlled Valorization of Xylose-derived Compounds by Metabolically Engineered Corynebacterium glutamicum
  • Synthetic Biology and Engineering 2023, 1(2), 10009; https://doi.org/10.35534/sbe.2023.10009
  • Lynn Schwardmann    Marielle Rieks   Volker F. Wendisch   Check
     31 May 2023

    The implementation of bioprocesses in an economically feasible and industrial competitive manner requires the optimal allocation of resources for a ba...

    Article Open Access
    Development of a New 1,2,4-butanetriol Biosynthesis Pathway in an Engineered Homoserine-producing Strain of Escherichia coli
  • Synthetic Biology and Engineering 2023, 1(1), 10007; https://doi.org/10.35534/sbe.2023.10007
  • Yujun Zhang   Lin Chen   Antu Thomas   An-Ping Zeng   Check
     10 May 2023

    1,2,4-butanetriol (BT) is a compound of high interest with applications in pharmaceutical and materials. In this work, we designed a novel biosynthe...

    Review Open Access
    Coiled Coils as Versatile Modules for Mammalian Cell Regulation
  • Synthetic Biology and Engineering 2023, 1(1), 10006; https://doi.org/10.35534/sbe.2023.10006
  • Estera Merljak   Anja Golob-Urbanc    Tjaša Plaper   Roman Jerala   Check
     06 Apr 2023

    Synthetic biology is a rapidly growing field that allows us to better understand biological processes at the molecular level, and enables therapeutic ...

    Article Open Access
    Fed-batch Self-regulated Fermentation of Glucose to Co-produce Glycerol and 1,3-propanediol by Recombinant Escherichia coli
  • Synthetic Biology and Engineering 2023, 1(2), 10008; https://doi.org/10.35534/sbe.2023.10008
  • Guimin Liu   Cai Feng   Zhiwei Zhu   Yaqin Sun   Zhilong Xiu   Check
     22 May 2023

    As important bio-chemicals, glycerol and 1,3-propanediol (1,3-PDO) have been widely used in numerous fields, e.g., polymers, cosmetics, foods, lubrica...

    Article Open Access
    Hydroxybenzoic Acid Production Using Metabolically Engineered Corynebacterium glutamicum
  • Synthetic Biology and Engineering 2023, 1(2), 10010; https://doi.org/10.35534/sbe.2023.10010
  • Misa Doke   Mayumi Kishida   Yuuki Hirata   Mariko Nakano   Mayo Horita   Daisuke Nonaka   Yutaro Mori   Ryosuke Fujiwara   Akihiko Kondo   Shuhei Noda   Tsutomu Tanaka   Check
     19 Jul 2023

    Hydroxybenzoic acids (HBAs), including 4-HBA, 3-HBA, and 2-HBA, are valuable platform chemicals for production of commodity materials and fine chemica...

    Review Open Access
    In Vitro BioTransformation (ivBT): Definitions, Opportunities, and Challenges
  • Synthetic Biology and Engineering 2023, 1(2), 10013; https://doi.org/10.35534/sbe.2023.10013
  • Yi-Heng P. Job Zhang   Zhiguang Zhu   Chun You   Lingling Zhang   Kuanqing Liu   Check
     01 Sep 2023

    Great needs always motivate the birth and development of new disciplines and tools. Here we propose in vitro BioTransformation (ivBT) as a ne...

    Review Open Access
    Challenging Post-translational Modifications in the Cell-free Protein Synthesis System
  • Synthetic Biology and Engineering 2023, 1(2), 10011; https://doi.org/10.35534/sbe.2023.10011
  • Kassidy B. Porche   Claire E. Lanclos   Yong-Chan Kwon   Check
     28 Jul 2023

    Post-translational modifications (PTMs) represent a cornerstone in the complexity of the proteome, significantly contributing to diversifying protein ...

    Commentary Open Access
    Synthetic Biology Industry in China: Current State and Future Prospects
  • Synthetic Biology and Engineering 2023, 1(2), 10014; https://doi.org/10.35534/sbe.2023.10014
  • Wei Luo   Yang Zhang   Jun Peng   Lishan Zhao   Check
     14 Sep 2023

    In this article, we provided an overview of the current state of the SynBio industry in China with a focus on its research and technology, its main ap...

    Review Open Access
    Dynamic Metabolic Control: From the Perspective of Regulation Logic
  • Synthetic Biology and Engineering 2023, 1(2), 10012; https://doi.org/10.35534/sbe.2023.10012
  • Tian Jiang   Chenyi Li   Yuxi Teng   Jianli Zhang   Diana Alexis Logan   Yajun Yan   Check
     28 Aug 2023

    Establishing microbial cell factories has become a sustainable and increasingly promising approach for the synthesis of valuable chemicals. However, i...

    Review Open Access
    Metabolic Engineering of Microorganisms Towards the Biomanufacturing of Non-Natural C5 and C6 Chemicals
  • Synthetic Biology and Engineering 2023, 1(3), 10015; https://doi.org/10.35534/sbe.2023.10015
  • Ashley Tseng   Vanna Nguyen   Yuheng Lin   Check
     31 Oct 2023

    Five-carbon (C5) and six-carbon (C6) chemicals are essential components in the manufacturing of a variety of pharmaceuticals, fuels, polymers, and oth...

    Article Open Access
    Time-efficient and Semi-automated Production and Screening of Proteins
  • Synthetic Biology and Engineering 2023, 1(3), 10016; https://doi.org/10.35534/sbe.2023.10016
  • S. R. Sekar   S. Ilhan   Uwe Jandt   An-Ping Zeng   Check
     18 Dec 2023

    Fast, flexible and non-randomized modification, production and screening of proteins in fully automated system are of high interest in biological rese...

    Article Open Access
    Serine Integrase-based Recombination Enables Direct Plasmid Assembly In Vivo
  • Synthetic Biology and Engineering 2023, 1(3), 10017; https://doi.org/10.35534/sbe.2023.10017
  • Luyao Wang   Yufei Zhang   Wan-Qiu Liu   Fang Ba   Jian Li   Check
     20 Dec 2023

    Serine integrases are emerging as one of the powerful tools for synthetic biology. They have been widely developed across genome engineering, biologic...

    Review Open Access
    Deciphering the Code of Pattern Formation: Integrating In Silico and Wet Lab Approaches in Synthetic Biology
  • Synthetic Biology and Engineering 2023, 1(3), 10018; https://doi.org/10.35534/sbe.2023.10018
  • Anqi Xu   Lizhong Liu   Jian-Dong Huang   Check
     27 Dec 2023

    Pattern formation is a fundamental process in biological development, enabling the transformation of initially uniform or random states into spatially...

    Article Open Access
    Analysis of a σ54 Transcription Factor L420P Mutation in Context of Increased Organic Nitrogen Tolerance of Photofermentative Hydrogen Production in Cereibacter sphaeroides Strain 2.4.1 Substrain H2
  • Synthetic Biology and Engineering 2024, 2(1), 10001; https://doi.org/10.35534/sbe.2024.10001
  • Nadine Wappler   Röbbe Wünschiers   Check
     23 Jan 2024

    Photofermentative hydrogen production with non-sulfur purple bacteria like Cereibacter sphaeroides (formerly Rhodobacter sphaeroides...