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One-pot Multi-enzyme Cascade Synthesis of Bifunctional Compounds from Vegetable Oils

Synthetic Biology and Engineering. 2024, 2(1), 10004; https://doi.org/10.35534/sbe.2024.10004
Xiaoxia Gao    Ran Lu    Yueyue Zhou    Lu Lin *    Xiao-Jun Ji *   
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, China
*
Authors to whom correspondence should be addressed.

Received: 17 Jan 2024    Accepted: 20 Feb 2024    Published: 21 Feb 2024   

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Abstract

Green and efficient biocatalytic technology has become a complementary or alternative means of organic synthesis. Chemicals with two functional groups, such as α,ω-dicarboxylic acids, ω-amino fatty acids and ω-hydroxy fatty acids, are widely used in the synthesis of polymers such as polyesters and polyamides. In recent years, the production of biodegradable materials using renewable and abundant vegetable oils as green raw materials has attracted increasing attention, receiving an additional impetus from synthetic biology. This paper presents the recent research progress in the production of bifunctional chemicals with medium chain lengths of C8–C12 using multi-enzyme cascades. Recent studies have developed multilevel optimization strategies to improve the efficiency, economics, and sustainability of multi-enzyme cascades. Cofactor regeneration strategies were developed to avoid large additions of expensive coenzymes. Protein engineering strategies were applied to improve enzyme stability and catalytic performance. In addition, blocking the β-oxidation pathway, improving the efficiency of substrate transport across membranes and increasing cellular robustness are effective optimization strategies for whole-cell catalytic systems. In addition, we discuss the development prospects of producing high value-added fine chemicals from vegetable oils using one-pot multi-enzyme reaction systems.

Keywords

Bifunctional compounds; Vegetable oils; One-pot multi-enzyme cascade reaction; Medium-chain fatty acids

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