Synthetic Biology and Engineering, Volume 1, Issue 1 (June 2023) – 7 articles

Cover Story (view full-size image):
Regulation of mammalian cells represents a showcase of synthetic biology, which is rapidly translated towards therapeutic and biotechnological applications. In accordance with the engineering principles of synthetic biology, designable modular protein building blocks, such as coiled coils, represent a powerful toolset for designed heterodimerization used to regulate diverse processes. Our review highlights the exceptional advantages of designable coiled-coil peptides, demonstrating their remarkable versatility. They have been used to specify cellular localization, regulate transcription, construct fast responsive systems and protein secretion, fostering cell-cell interactions, and biological applications from CAR T cell regulation to genome editing.
Article Open Access
Production of Highly Modified C30-carotenoids with Singlet Oxygen-quenching Activities, 5-glucosyl-5,6-dihydro-4,4’-diapolycopen-4’-oic Acid, and Its Three Intermediates Using Genes from Planococcus maritimus Strain iso-3
  • Synthetic Biology and Engineering 2023, 1(1), 10002;
  • Moe Hagiwara   Chinatsu Maehara   Miho Takemura   Norihiko Misawa   Kazutoshi Shindo   Check
     07 Feb 2023

    Planococcus maritimus strain iso-3 was previously isolated from intertidal sediment in the North Sea and was found to produce a highly modifie...

    Review Open Access
    Increasing Nutritional Value of Cyanobacteria by Engineering Valine, Phenylalanine, and Fatty Acid Production
  • Synthetic Biology and Engineering 2023, 1(1), 10003;
  • Nick Lopez-Riveira   Stephen Fong   Check
     16 Feb 2023

    In 2020, the United Nations estimated that 2.37 billion people globally were without food or unable to eat a healthy balanced diet. The number of peop...

    Article Open Access
    Design of Oscillatory Networks through Post-translational Control of Network Components
  • Synthetic Biology and Engineering 2023, 1(1), 10004;
  • Brianna E.K. Jayanthi   Shridhar Jayanthi   Laura Segatori   Check
     13 Mar 2023

    Many essential functions in biological systems, including cell cycle progression and circadian rhythm regulation, are governed by the periodic behav...

    Review Open Access
    Thermoanaerobacter Species: The Promising Candidates for Lignocellulosic Biofuel Production
  • Synthetic Biology and Engineering 2023, 1(1), 10005;
  • Kaiqun Dai   Chunyun Qu   Hongxin Fu   Jufang Wang   Check
     15 Mar 2023

    Thermoanaerobacter species, which have broad substrate range and high operating temperature, can directly utilize lig...

    Review Open Access
    Coiled Coils as Versatile Modules for Mammalian Cell Regulation
  • Synthetic Biology and Engineering 2023, 1(1), 10006;
  • Estera Merljak   Anja Golob-Urbanc    Tjaša Plaper   Roman Jerala   Check
     06 Apr 2023

    Synthetic biology is a rapidly growing field that allows us to better understand biological processes at the molecular level, and enables therapeutic ...

    Article Open Access
    Development of a New 1,2,4-butanetriol Biosynthesis Pathway in an Engineered Homoserine-producing Strain of Escherichia coli
  • Synthetic Biology and Engineering 2023, 1(1), 10007;
  • Yujun Zhang   Lin Chen   Antu Thomas   An-Ping Zeng   Check
     10 May 2023

    1,2,4-butanetriol (BT) is a compound of high interest with applications in pharmaceutical and materials. In this work, we designed a novel biosynthe...