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Article

17 June 2024

Cytosine Deaminase-Assisted Mutator for Genome Evolution in Cupriavidus necator

Cupriavidus necator H16 has been intensively explored for its potential as a versatile microbial cell factory, especially for its CO2 fixation capability over the past few decades. However, rational metabolic engineering remains challenging in the construction of microbial cell factories with complex phenotypes due to the limited understanding of its metabolic regulatory network. To overcome this obstacle, laboratory adaptive evolution emerges as an alternative. In the present study, CAM (cytosine deaminase-assisted mutator) was established for the genome evolution of C. necator, addressing the issue of low mutation rates. By fusing cytosine deaminase with single-stranded binding proteins, CAM introduced genome-wide C-to-T mutations during DNA replication. This innovative approach could boost mutation rates, thereby expediting laboratory adaptive evolution. The applications of CAM were demonstrated in improving cell factory robustness and substrate utilization, with H2O2 resistance and ethylene glycol utilization as illustrative case studies. This genetic tool not only facilitates the development of efficient cell factories but also opens avenues for exploring the intricate phenotype-genotype relationships in C. necator.

Commentary

17 June 2024

Unraveling Novel Strategies: Targeting Miz1 for Degradation to Enhance Antiviral Defense against Influenza A Virus

The ubiquitin system has been shown to play an important role in regulation of immune responses during viral infection. In a recent article published in Science Signaling, Wu and colleagues revealed that transcriptional factor Miz1 plays a pro-viral role in influenza A virus (IAV) infection by suppressing type I interferons (IFNs) production through recruiting HDAC1 to ifnb1 promoter. They show that a series of E3 ligases combinatorially regulates Miz1 ubiquitination and degradation and modulates IFNs production and viral replication.

Editorial

14 June 2024

Review

14 June 2024

Biobased Vitrimers: A Sustainable Future

Vitrimers are crosslinked polymers containing dynamic covalent linkages. Because of their crosslinked structure, they are stable as thermosets at their service temperatures. At high enough temperatures, dynamic exchange reactions occur and rearrange the polymer network, thus vitrimers become malleable and reprocessable like thermoplastics. The dynamic covalent bonds can also undergo dissociative cleavage reactions under specific conditions, so vitrimers are inherently degradable. To achieve a sustainable future, various biomass resources have been used as raw materials in vitrimer preparation. This review summarizes recent developments in biobased vitrimers and highlights their preparation methods. The limitations of current biobased vitrimers are also discussed.

Article

13 June 2024

Optimizing Performance and Design Simulation of a 100 KW Single Rotor Horizontal Axis Wind Turbine

As wind energy becomes increasingly vital in global energy strategies, optimizing wind turbine design is essential. This research focuses on the development of a 100 kW single rotor horizontal axis wind turbine (HAWT) tailored to meet the energy needs of Jamshoro, Pakistan. The turbine design leverages SolidWorks for structural modeling and is validated through comprehensive simulations using ANSYS and Q-Blade. Operating at an optimal wind speed of 6.9 m/s, the turbine achieves maximum efficiency, as indicated by the highest power factor. This efficiency translates to an estimated power output of approximately 100 kW, suitable for common household consumption. The study integrates regional climatic data and wind conditions to enhance turbine performance and durability. The findings offer a sustainable energy solution for Jamshoro, contributing to Pakistan’s renewable energy infrastructure and addressing local energy demands effectively. The focus of this study will be Jamshoro, a region in Pakistan as a case study. The software simulations will consider a variety of elements, including as wind speeds, variable loads, and environmental factors unique to the chosen region (Jamshoro). This research proposes a sustainable solution for addressing the energy demands in Jamshoro by integrating accurate data based on software analysis with real-world concerns, adding to the larger goal of developing sustainable sources of energy in Pakistan.

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