This article presents conceptual and methodological reflections that have emerged from a participatory action research project in the binational Lake Titicaca region. The ecosystem faces critical degradation due to mining contamination and untreated wastewater, which has led to the establishment of a series of local initiatives, as the recent recognition of the lake as a rights holder in Peru. In this spectrum, the research sought to bolster local defense initiatives by facilitating internal spaces for dialogue and co-production of knowledge, and by exploring avenues for strengthening collective strategies to transform water-related conflicts. Central to this study is the “Dialogue of Water Stories”, a community-based methodological proposal that integrates theoretical and practical components of dialoguing and storytelling. The findings demonstrate that this methodology effectively articulates the discussion of conflicts, unpacking several perspectives from multiple stakeholders. In this case, this led to the revelation of a plurality of community water values and historical care practices—particularly those upheld by women—while generating resonance for regional water defense. The article proposes the “Dialogue of Water Stories” as a transformative methodological approach to narrating water struggles and inspiring socio-environmental change.
Multilayer composite materials, having high specific strength and rigidity, are sensitive to interlayer defects. The problem of interlayer laminations in a composite plate subjected to a plane compressive load is studied using a new analytical structure previously developed by the authors. Elastic characteristics of a multilayer package of thin lamination, including the elastic characteristics of separate layers, depending on modulus of elasticity, shear modulus, Poisson’s ratio, and angle of orientation of fibers of the unidirectional layer, are determined. Ratios are obtained for the unidirectional composite material that reflect the contribution of each component (fiber, matrix) in proportion to its volume fraction, according to the so-called “mixture rule”. This work examines the behavior after the loss of stability of an elliptical defect in a composite plate. Only the local bulging of the delamination type defect was considered. The difference between this work and others lies in the fact that the application of the developed method, based on the energy approach, makes it possible to obtain explicit analytical expressions for quantities characterizing the critical load and describing the supercritical behavior of the detached part. The energy method is generalized to the case of analyzing the stability of defects in a non-linear formulation. The value of the critical load was obtained, and the analysis of the supercritical deformation of the defect was made.
Precise labeling of alveolar type 2 (AT2) cells is essential for elucidating lung development and injury responses. In this study, we evaluated Abca3 and Etv5-based genetic strategies for labeling AT2 cells in murine models. Using targeted genetic approaches, we generated Abca3-rtTA and Etv5-rtTA knock-in mouse lines and crossed them with pTRE-H2BGFP to create inducible reporter models driven by Abca3 or Etv5. Labeling specificity and efficiency were assessed by flow cytometry and co-immunostaining. Our results show that both Abca3 and Etv5 strategies faithfully label AT2 cells across developmental stages and following lung injury. Comprehensive analyses confirmed the high specificity and efficiency of labeling. These Abca3- and Etv5-driven systems offer robust tools for investigating AT2 cell biology and pathology and may serve as effective drivers for tetO-mediated gene knockout or overexpression studies specifically in AT2 cells in mouse models.
Lung microvascular endothelial inflammation and barrier dysfunction play critical roles in the pathogenesis of acute lung injury (ALI)/acute respiratory distress syndrome (ARDS). Despite recent scientific advances, the mortality of ALI/ARDS is still extremely high because the molecular mechanisms involved in ALI/ARDS remain unclear. In a recent issue of the journal Advanced Science, Baoyinna and colleagues reported that deubiquitinase USP30 induces lung microvascular inflammation and endothelial barrier disruption through the S-adenosylmethionine (SAM) cycle, DNA methylation, and miR-30a-5p down-regulation in ALI/ARDS. Their findings provide a strong rationale for targeting microRNAs, S-adenosylmethionine, DNA methylation, and deubiquitinating enzymes as potential therapeutic strategies for the treatment of ALI/ARDS.
Greece confronts intensifying water scarcity driven by population growth, urbanization, tourism, and climate variability, despite its extensive coastline. Traditional sources are strained, with agriculture consuming ~80% of withdrawals (surface water ~38%, groundwater ~62%). Desalination, predominantly reverse osmosis (RO), offers a mature solution, already meeting 30–95% of domestic needs in Aegean islands, but its energy intensity challenge sustainability within the water–energy–food nexus. This study presents a geospatial framework to assess energy requirements for a hypothetical scenario in which seawater desalination fully supplies domestic water demand in Greece. High-resolution GIS data, WorldPop population grids, and hydrological networks enable estimation of daily demand (173 L/capita/day) and energy decomposition: desalination (SEC = 5 kWh/m3 SWRO), elevation pumping plus residual pressure (15 m head), and frictional losses. The hypothetical pipelines follow reverse natural drainage paths for realistic routing. Results highlight substantial spatial disparities: inland cities face significantly higher and more uniform energy costs (Ioannina: mean dynamic head 8.3 kWh/m3, ~43% higher than the coastal reference of Athens at 5.8 kWh/m3), driven by elevation and distance; coastal centres show lower means but greater variability (Athens: highest total ~3.35 GWh/day). In summary, fully supplying domestic water demand via desalination would necessitate an additional ~8% of the country’s total electricity consumption. Findings affirm desalination’s potential for coastal/island supply while revealing energy barriers inland.
