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Development and Perspective of Production of Terpenoids in Yeast

Synthetic Biology and Engineering. 2024, 2(1), 10003; https://doi.org/10.35534/sbe.2024.10003
Yaying Xia ,†    Congna Li ,†    Ruidi Cao    Lu Qin    Shuobo Shi *   
Beijing Advanced Innovation Center for Soft Matter Science and Engineering, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
†
Yaying Xia and Congna Li contributed equally.
*
Authors to whom correspondence should be addressed.

Received: 05 Jan 2024    Accepted: 02 Feb 2024    Published: 13 Feb 2024   

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Abstract

Terpenoids are a large class of secondary metabolites known for their remarkable diverse biological activities, making them widely utilized in the pharmaceutical, food, cosmetic, biofuel and agricultural fields. However, the current production of terpenoids heavily relies on plant extraction and chemical synthesis, which brings about concerns regarding infield, environmental and ecological issues. With the advancements in metabolic engineering and emerging synthetic biology tools, it is now possible to sustainably produce these high value-added terpenoids using microbial chassis. Among them, yeast has emerged as a promising candidate for the heterologous biosynthesis of terpenoids due to its inherent advantages, including robustness, safety, and the availability of sufficient precursor. This review focuses on the diverse strategies employed to enable terpenoids production in yeasts. These strategies encompass metabolic engineering approaches to optimize the mevalonate pathway, protein engineering techniques to improve terpenoid biosynthesis, the applications of organelles compartmentalization, high throughput screening and global approaches for the development of efficient cell factories. Furthermore, this review discusses the future prospects and challenges associated with yeast-based terpenoid production, while also emphasizing guidelines for future studies in this field.

Keywords

Terpenoids; Metabolic engineering; Protein engineering; Subcellular compartments; Global approaches; Yeast

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