Artiles
Open Access
Article
09 December 2025Demethylation of Lignin from Rice-Straw Biorefinery: An Integrated Chemical and Biochemical Approach
The efficiency of lignocellulosic biorefineries is limited because of the high recalcitrance and low reactivity of lignin. The reactivity of lignin can be enhanced through various chemical and biochemical approaches. Demethylation is one of the methods that improve the availability of phenolic hydroxyl groups in lignin, thereby enhancing its reactivity and application in sustainable adhesives. The goal of this study is to integrate microbial and chemical approaches to aid in the demethylation of lignin. Towards that end, lignin was first extracted and purified from the rice straw biorefinery solid residue obtained post ethanol fermentation. This rice straw lignin was then subjected to chemical and microbial demethylation. For microbial demethylation under alkaline conditions, Pseudomonas putida and Pseudomonas fluorescens were employed, while demethylation under neutral conditions was conducted using Trametes versicolor. Integrated treatment using Pseudomonas putida followed by hydrogen iodide yielded an increase in the phenolic hydroxyl content by approximately 39–43%. Demethylation using chemical methods and biological methods alone provided approximately 18–27% increases in phenolic hydroxyl content, respectively. Furthermore, to assess the physical and chemical properties of demethylated lignin, FT-IR, TGA, and morphological analytical tools were employed.
Open Access
Article
09 December 2025Defiant Doctorates: Reshaping the Social, Cultural and Intellectual Value of the Doctorate in Regional Universities
Universities are ranked and clustered into ‘like-minded’ institutions. Regional universities—as an adjective and noun or a compound noun—are defined via location, rather than academic standards, teaching innovation, research rigour, or the use of innovative technology. Through the ‘regional’ labelling, they are marked and separated as different from, and implicitly less than, urban and metropolitan institutions, which carry the excitement of urbanity, encompassing Virilian speed and prestigious alumni. This differentiation has consequences for grants, funding, academic staff attrition, and leadership. But what happens to PhD students at regional universities? Where is their voice? How are their views recognized, codified, and understood? Written between an experienced supervisor and a PhD student, this paper offers a different pathway through the regional graduate programme, offering a different lens to re-vision regional higher education, beyond cliches of partnerships and collaborations. As a theoretical and conceptual paper, it creates and holds space for PhD students in a revisioning of regional universities.
Open Access
Article
08 December 2025Short-Form Video Application Use and Self-Rated Health among Older Adults: The Mediating Role of Perceived Social Support and the Moderating Role of Media Literacy
Although short-form video applications (apps) are increasingly popular among older adults, little research has investigated the relationship between their use of such apps and health outcomes. The present study aims to investigate this relationship while examining the mediating role of perceived social support and the moderating role of media literacy. Three hundred and nineteen older adults completed our questionnaire. The results showed that short-form video app use was positively associated with self-rated health among older adults, and this association was mediated by perceived social support. Moreover, the positive association between short-form video app use and perceived social support was observed in older adults with lower (but not higher) levels of media literacy. Future interventions aimed at promoting the health and well-being of older adults (especially those with lower media literacy) should consider teaching participants to use short-form video apps appropriately.
Open Access
Review
08 December 2025Advances in the Biosynthesis and Application of Sialyllactose
Human milk oligosaccharides (HMOs), the third most abundant solid component in human milk after lactose and lipids, are recognized as crucial prebiotics that support infant gut health and immune development. Salivary HMOs account for approximately 13% of the total HMOs molar ratio, with 3′-sialyllactose (3′-SL) and 6′-sialyllactose (6′-SL) accounting for approximately 2% and 6%, respectively. Sialyllactose (SL) exhibits a range of notable physiological functions, including prebiotic activity, antiviral properties, prevention of necrotizing enterocolitis, immunomodulatory effects, and enhancement of brain development and cognition. Both 3′-SL and 6′-SL have been approved as “Generally Recognized as Safe” (GRAS) by the U.S. FDA and are increasingly incorporated into infant formula. Currently, the biosynthesis of SL is mainly efficiently produced through engineered microorganisms. However, face bottlenecks: low yields, complex downstream processing, and prohibitive costs. Recent advances in synthetic biology and metabolic engineering offer promising avenues to overcome these barriers. This review introduces the synthesis methods, functions, and applications of SL, as well as conducting safety evaluation and regulatory status analysis. We hope this article will enhance understanding of the challenges encountered in the synthetic biology production and application of SL.
Open Access
Article
08 December 2025Public Participation in Ecological Civilization Construction in Urumqi: A Case Study of a Rapidly Expanding Arid Metropolis in Northwestern China
Public participation in ecological civilization construction is a critical pathway for advancing ecological urban design. This study examines residents’ perceptions, satisfaction, and participation in the construction of ecological civilization in Urumqi, northwestern China. Drawing on 1012 questionnaires, this empirical study investigates factors influencing public participation in the construction of ecological civilization. The findings indicate that residents exhibited a strong subjective awareness of public participation in ecological civilization construction (mean score = 4.66), yet ecological cognition (2.75) and participation confidence (2.97) were relatively weak and require further improvement. Satisfaction levels were relatively higher for green status (2.51) and information transparency (2.41), whereas overall satisfaction remained modest, with water resources (1.81) and waste management (1.99) emerging as key concerns. Residents demonstrated a moderate willingness to contribute financially and primarily engaged in low-cost, habitual ecological practices. Significant differences were observed across socio-demographic variables (p < 0.05). Uncivil behaviors and natural pressures were observed as visible obstacles. Strong government leadership, active public engagement, and effective media communication contribute to advancing ecological civilization construction. These results provide valuable insights for promoting ecological civilization construction in northwestern China.
Open Access
Article
05 December 2025Long-Term Creep Performance of Ferritic SOC Interconnect Steel
Crofer® 22 H is a well-known commercial ferritic stainless steel for the construction of SOC interconnect plates. Its performance under creep loading conditions in the temperature range from 700 to 800 °C was evaluated against a pre-commercial trial steel to signify the impact of thermomechanical treatment history on long-term creep response. While the commercial grade prevailed in solution treated, i.e., low dislocation density, state, the trial steel was put into creep service in a deformed, i.e., high dislocation density, condition. Dislocations do play a major role in the early stages of the nucleation of strengthening Laves phase precipitates, and for this reason, sensitively impact the creep response of the materials in the primary stage of creep, which even affects the following (limited) secondary stage and especially the transition into the creep life dominating tertiary creep stage.
Open Access
Article
05 December 2025Stopping Rules for Two-Sigma Structural Monophyly in Morphology-Based High-Resolution Phylogenetics
Stopping rules for sampling designs are critical for limiting the effort needed to obtain adequate or significant data, and in many cases for conservation of the species sampled. Such rules are commonly based on pre-determined criteria or a lack of new information as sampling continues. Structural monophyly analysis of minimally monophyletic groups of one ancestral species and a few immediate ancestral species uses a series of steps, each step with a statistical evaluation that helps produce a concise model. Demonstration of two-sigma exclusion of uncertainty is a new stopping rule requirement. The full series of analytic steps has not previously been consolidated in one publication.
Open Access
Article
04 December 2025Stakeholder Mental Models for Sustainable Management of the Invasive Pearl Oyster Pinctada radiata in the Eastern Mediterranean
Sustainable management of marine and coastal systems depends not only on ecological dynamics but also on the ways stakeholders perceive and interpret them. This study investigates how fishers, scientists, and government officials understand and frame the management of the Indo-Pacific pearl oyster Pinctada radiata, a non-native yet economically valuable species established around Evia Island, Greece. Using a mixed-methods approach (N = 80), we combined an eleven-item Hydro-ecological Governance Perception Scale (HGPS) with open-ended responses to explore cognitive patterns and governance perspectives. Sampling adequacy was satisfactory (KMO = 0.74; Bartlett’s χ2(55) = 350.41, p < 0.001) and factor analysis revealed two interrelated dimensions explaining 67.8% of total variance (α = 0.84; ω = 0.86; CR = 0.82). Although Kruskal–Wallis tests showed no statistically significant differences among groups (p > 0.05), hierarchical clustering distinguished three partially overlapping cognitive profiles: Ecological Pragmatists, Institutional Collaborators, and Adaptive Stewards (Silhouette = 0.45; CH = 150.23; DBI = 0.75). Thematic and sentiment analyses underscored the importance of collaboration, transparency, and education (mean sentiment = 0.58). The findings demonstrate how cognitive diversity can improve hydro-ecological resilience and the sustainability of coastal governance when it is mobilized through co-management and participatory monitoring.
Open Access
Article
02 December 2025The Role of Electric Vehicles in Environmental Transformation-Goal Towards a Pollution-Free Climate
Government agencies have worked tirelessly to minimize the effects of pollution. This problem is pretty dominant in developing countries like Pakistan. The world is facing a severe problem in the form of pollution and the greenhouse effect in recent years. At present, cities like Karachi and Lahore are facing a very high index of Air pollution caused by vehicular emissions. In this framework, the current research proposes an optimized design of student electric vehicles to attenuate environmental pollution. Electric vehicles produce zero tailpipe emissions, which means no toxic gases. A Carbon Footprint Analysis is conducted in the proposed study to measure the effect of greenhouse gases over the various phases of a vehicle’s life. To simulate the long-term impacts of electric vehicles on the environment, Agent-based Modelling is performed. It mainly includes the analysis of technological advancements in battery recycling. The idea of the student electric vehicle is based on several key points, including the use of an AGS (Automatic Gear System) and Self-Driving mode, to make it easier for students to navigate. Further, a sensing mechanism is developed for predictive maintenance and diagnostics. Hence, the proposed idea of student electric vehicles may be a game-changer for the students by providing them with a safe and pollution-free environment. The analysis shows that EVs like those proposed by students will reduce life cycle emissions by upto 71 percent as compared to ICE.
Open Access
Article
01 December 2025Preparation, Characterization and Performance Assessment of Metal Complexes of Curcuma longa Extract as Sensitizers for Dye-Sensitized Solar Cells
The dye extract of Curcuma longa (turmeric), which is very rich in curcumin, was chemically modified by complexation reaction with Zn2+, Cu2+, and Fe3+ ions to enhance its stability, electron transfer and photovoltaic performance. The dye and complexes were characterized by Ultraviolet-Visible (UV-Vis) absorption and Fourier Transform Infra-Red (FTIR) spectroscopy of potential chromophores and functional groups. The spectral data obtained indicated that the curcuminoid ligands were successfully coordinated with the metal centers, resulting in red-shifted absorption bands from beyond 460 nm and C=O vibrational frequency decreasing below 1650 cm−1. Complexation reaction resulted in improved photochemical response and enhanced light-harvesting potential. When compared, the solar cells fabricated with titanium dioxide (TiO2) photoanodes sensitized by the complexes afforded improvement in the magnitude of short-circuit current density as well as power conversion efficiency compared to the devices sensitized with the crude extract. Among the three complexes, the Zn-complex afforded the highest efficiency (1.20%), attributed to favourable electronic coupling and reduced recombination losses. Computational studies conducted through quantum chemical calculations based on the curcumin structure supported the experimental findings. The findings from this study demonstrate that metal ions-natural dye complexes have potential for application as low-cost, eco-friendly and sustainable sensitizers, thereby opening a novel horizon in green photovoltaic technologies.
