Artiles
Communication
21 March 2023Established Hepatic Stellate Cell Lines in Hepatology Research
Hepatic stellate cells comprise a minor cell population in the liver, playing a key role in the pathogenesis of hepatic fibrosis. In chronic liver damage, these cells undergo a transition from a quiescent to a highly proliferative phenotype with the capacity to synthesize large quantities of extracellular matrix compounds such as collagens. Because of their pivotal role in liver disease pathogenesis, this hepatic cell population has become the focus of liver research for many years. However, the isolation of these cells is time consuming and requires the trained laboratory personnel. In addition, working with primary cells requires the following of ethical and legal standards and potentially needs the approval from respective authorities. Therefore, continuous growing hepatic stellate cells have become very popular in research laboratories because they are widely available and easy to handle, and allow a continuous supply of materials, and further reduction of lab animal use in biomedical research. This communication provides some general information about immortalized hepatic stellate cell lines from mouse, rats and humans.
Review
15 March 2023Ultra-Thin Solid Electrolyte in Lithium-Ion Batteries
Safety concern of lithium-ion battery, attributed to using volatile and flammable liquid electrolytes, could be addressed by using solid electrolytes. Solid electrolytes including inorganic solid electrolytes, polymer solid electrolytes and organic/inorganic composite electrolytes have the common drawbacks in low ion-conductivity. Much efforts have been devoted to increase the specific ion conductivity, especially for inorganic solid electrolyte whose intrinsic conductivity is close to liquid electrolyte. However, most solid-state electrolyte membranes in lithium-ion batteries are thick, resulting in long ion-conduction pathway, low energy density and high cost. In this review, the advantages and disadvantages of different kinds of solid electrolytes were analyzed, and the promising strategies of ultra-thin solid electrolyte preparation were summarized and prospected. Applied organic-inorganic composite, continuous phase enhancement and in situ integration have been devoted to reducing thickness of electrolyte membrane and improving battery performance. On the basis of the technical requirement of lithium-ion batteries, this review aims to provide a guidance in terms of rational design and synthesis of ultra-thin solid electrolytes for the further research that addresses the safety issues and improves cycling performance of batteries.
Review
15 March 2023Thermoanaerobacter Species: The Promising Candidates for Lignocellulosic Biofuel Production
Thermoanaerobacter species, which have broad substrate range and high operating temperature, can directly utilize lignocellulosic materials for biofuels production. Compared with the mesophilic process, thermophilic process shows greater prospects in consolidated bioprocessing (CBP) due to its relatively higher efficiency of lignocellulose degradation and lower risk of microbial contamination. Additionally, thermophilic conditions can reduce cooling costs, and further facilitate downstream product recovery. This review comprehensively summarizes the advances of Thermoanaerobacter species in lignocellulosic biorefinery, including their performance on substrates utilization, and genetic modification or other strategies for enhanced biofuels production. Furthermore, bottlenecks of sugar co-fermentation, metabolic engineering, and bioprocessing are also discussed.
Article
13 March 2023Reduced Climate Impacts of Dairy Sludge Management by Introducing Hydrothermal Carbonization
Dairies which produce cheese and milk products can, however, produce large volumes of wastewater that require treatment, usually via activated sludge treatment. Disposal of the resulting activated sludge to land is viewed favorably as the sludge is rich in phosphorus (P) and nitrogen (N) and enables nutrient recycling. Nonetheless, sludge management can significantly influence the greenhouse gas (GHG) emissions to the atmosphere. This manuscript has modelled the GHG emissions arising from two sludge management strategies currently adopted by Danish dairies whereby: (i) sludge is stored and later applied to fields; or (ii) sludge is treated by anaerobic digestion (AD), stored, and the digestate will later be applied to fields. This is compared to (iii) an alternative sludge management strategy with treatment by Hydrothermal Carbonization (HTC). HTC is a technologically simple sludge treatment that could lower the cost for dewatering dairy sludge, forming a biochar-like material known as hydrochar. The produced hydrochar can be applied to the land for the purpose of carbon sequestration, P and N recycling. Our calculations indicate that GHG balances of HTC sludge management can result in a net carbon sequestration of 63 kg CO2eq per ton sludge, as opposed to net emissions of 420 and 156 kg CO2eq per ton sludge for strategies (i) and (ii), therefore offering significant reductions GHG emissions for the dairy sector.
Article
13 March 2023Design of Oscillatory Networks through Post-Translational Control of Network Components
Many essential functions in biological systems, including cell cycle progression and circadian rhythm regulation, are governed by the periodic behaviors of specific molecules. These periodic behaviors arise from the precise arrangement of components in biomolecular networks that generate oscillatory output signals. The dynamic properties of individual components of these networks, such as maturation delays and degradation rates, often play a key role in determining the network's oscillatory behavior. In this study, we explored the post-translational modulation of network components as a means to generate genetic circuits with oscillatory behaviors and perturb the oscillation features. Specifically, we used the NanoDeg platform—A bifunctional molecule consisting of a target-specific nanobody and a degron tag—to control the degradation rates of the circuit’s components and predicted the effect of NanoDeg-mediated post-translational depletion of a key circuit component on the behavior of a series of proto-oscillating network topologies. We modeled the behavior of two main classes of oscillators, namely relaxation oscillator topologies (the activator-repressor and the Goodwin oscillator) and ring oscillator topologies (repressilators). We identified two main mechanisms by which non-oscillating networks could be induced to oscillate through post-translational modulation of network components: an increase in the separation of timescales of network components and mitigation of the leaky expression of network components. These results are in agreement with previous findings describing the effect of timescale separation and mitigation of leaky expression on oscillatory behaviors. This work thus validates the use of tools to control protein degradation rates as a strategy to modulate existing oscillatory signals and construct oscillatory networks. In addition, this study provides the design rules to implement such an approach based on the control of protein degradation rates using the NanoDeg platform, which does not require genetic manipulation of the network components and can be adapted to virtually any cellular protein. This work also establishes a framework to explore the use of tools for post-translational perturbations of biomolecular networks and generates desired behaviors of the network output.
Review
13 March 2023Review on Multi-Functional Separator for Li-S Batteries
Because lithium-ion batteries cannot meet increasing demand for power density, lithium metal batteries are expected as the next generation of rechargeable batteries. As one of lithium metal batteries, lithium-sulfur (Li-S) batteries have attracted extensive attention because of their ultrahigh power density (2600 Wh kg−1) and low cost of sulfur. In order to overcome problems of active material loss, volume expansion and dendritic growth of Li metal in Li-S batteries, researchers have adopted several methods such as adding electrolyte additives, electrode modification and separator modification. Among them, separator modification shows significant advantages in inhibiting the shuttle effect of lithium polysulfides. This paper reviews research progress of inhibiting the shuttle effect of Li-S batteries by the means of the separator modification in recent years, including direct design of new type of separator and physical/chemical modification of separator surface. Through extensive reading and summarizing of the research results of the separator modification of Li-S batteries, we give the possible development direction of Li-S batteries at the end of the paper.
Perspective
07 March 2023Pulsed Ultraviolet C as a Potential Treatment for COVID-19
Currently, low dose radiotherapy (LDRT) is being tested for treating life-threatening pneumonia in COVID-19 patients. Despite the debates over the clinical use of LDRT, some clinical trials have been completed, and most are still ongoing. Ultraviolet C (UVC) irradiation has been proven to be highly efficient in inactivating the coronaviruses, yet is considerably safer than LDRT. This makes UVC an excellent candidate for treating COVID-19 infection, especially in case of severe pneumonia as well as the post COVID-19 pulmonary fibrosis. However, the major challenge in using UVC is its delivery to the lungs, the target organ of COVID-19, due to its low penetrability through biological tissues. We propose to overcome this challenge (i) by using pulsed UVC technologies which dramatically increase the penetrability of UVC through matter, and (ii) by integrating the pulsed UVC technologies into a laser bronchoscope, thus allowing UVC irradiation to reach deeper into the lungs. Although the exact characteristics of such a treatment should yet to be experimentally defined, this approach might be much safer and not less efficient than LDRT.
Article
03 March 2023Spatial Characteristics and Influencing Factors of Migration in Kanagawa, Japan
In the context of “Tokyo centralization”, population migration has become an important factor affecting Kanagawa's economic growth, living standards, and employment status. On the other hand, with the development of the declining birth rate and aging society, migration for any purpose has an impact on social development. The government has released many policies to attract people from other cities to Kanagawa. This study analyzes the factors influencing the spatial pattern of population migration in Kanagawa based on the current spatial characteristics of population migration in Kanagawa from 2016~2020 and previous population migra-tion research theories. the influencing factors are analyzed empirically by selecting a total of 9 economic, social, and environmental indicators that may affect the spatial pattern of population migration in Kanagawa. The result showed that, when only the economic factor was considered, gross prefectural product, job opportunities, and consumer price index significantly influenced migration; When only environmental factors are considered, the number of pollution complaints successfully handled had a significant positive effect on population migration; When only the social environment is considered, the level of education becomes the main consid-eration for people. Furthermore, when the economic factors, environmental factors, and social factors are analyzed together, the gross prefectural domestic product, job opportunities, consumer price index, and the number of pollution complaints successfully handled all have an impact on migration in Kanagawa and the gross prefectural product is the common influencing factor.
Article
03 March 2023SnS2 Quantum Dots Decorated MoS2 Nanosheets Enabling Efficient Photocatalytic H2 Evolution in CO2 Saturated Water
SnS2/MoS2 heterojunction nanocomposite was prepared by a one-step hydrothermal synthesis method. The nanocomposite exhibited much improved photocatalytic hydrogen evolution performance in CO2 saturated solution compared with pure MoS2 and SnS2 samples. The improved photocatalytic activity was attributed to the S-scheme heterojunction structure between SnS2 quantum dots and MoS2 nanosheets which facilitate electron-hole separation both in MoS2 and SnS2. In the S-scheme structure, the strong reduction ability of SnS2 quantum dots was well maintained for the improved H2 evolution. In situ DRIFT studies allowed us to suggest reaction pathways from CO2 and H2O to photocatalytic H2, CO, and CH4 generation.
Communication
03 March 2023Evaluating Different UAS Flight Methods for 3D Model Generation and Printing of a Tornado Destroyed Cultural Heritage: Caddo House in Texas
In recent years, the use of Unmanned Aerial Systems (UAS) to obtain imagery for photogrammetry has become commonplace. Using these data to develop 3D products has also grown significantly in both research and commercial applications. This study aims to find a relatively simple and low cost UAS flight method as a means to obtain data to produce a 3D model suitable for 3D printing. The study subject chosen to assess different flight methods was the Caddo House at Caddo Mounds State Historical Site located near Alto, Cherokee County, Texas, USA. To collect images for analysis, a DJI Phantom 4 Pro UAS was used with Pix4DCapture mission control app. Two main missions were carried out, one being a pre-defined double-grid flight, and the other being an orbital free-flight method. The findings of this study indicate that if the goal is to create a true-to-life 3D model of an object using UAS, the best method would be a curated orbital free-flight method. If there is time constraint and the subject is sufficiently large and not considerably irregular, a double-grid mission with sufficient forward and side overlap can produce desirable results, but with a slight loss of fine details. The 3D model developed from the curated orbital flight method was successfully printed with a customer grade FDM 3D printer.
