Stopping Rules for Two-Sigma Structural Monophyly in Morphology-Based High-Resolution Phylogenetics

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Stopping Rules for Two-Sigma Structural Monophyly in Morphology-Based High-Resolution Phylogenetics

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Missouri Botanical Garden, 4344 Shaw Blvd., St. Louis, MI 63110, USA
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Received: 17 October 2025 Revised: 18 November 2025 Accepted: 24 November 2025 Published: 05 December 2025

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© 2025 The authors. This is an open access article under the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/).

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Ecol. Divers. 2025, 2(4), 10013; DOI: 10.70322/ecoldivers.2025.10013
ABSTRACT: Stopping rules for sampling designs are critical for limiting the effort needed to obtain adequate or significant data, and in many cases for conservation of the species sampled. Such rules are commonly based on pre-determined criteria or a lack of new information as sampling continues. Structural monophyly analysis of minimally monophyletic groups of one ancestral species and a few immediate ancestral species uses a series of steps, each step with a statistical evaluation that helps produce a concise model. Demonstration of two-sigma exclusion of uncertainty is a new stopping rule requirement. The full series of analytic steps has not previously been consolidated in one publication.
Keywords: Cladistics; Entropy; Evolution; Minimally monophyletic group; Monophyly; High-resolution phylogenetics; Stopping rules; Two-sigma support
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