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Analysis of a σ⁵⁴ Transcription Factor L420P Mutation in Context of Increased Organic Nitrogen Tolerance of Photofermentative Hydrogen Production in Cereibacter sphaeroides Strain 2.4.1 Substrain H2

Photofermentative hydrogen production with non-sulfur purple bacteria like Cereibacter sphaeroides (formerly Rhodobacter sphaeroides) is a promising and sustainable process to convert organic waste into the energy carrier hydrogen gas. However, this conversion is inhibited by elevated organic nitrogen concentrations in the substrate, which limits its applicability to nitrogen-poor organic waste. We present genomic and transcriptomic insights into a substrain of Cereibacter sphaeroides strain 2.4.1 that shows unexpected high levels of photofermentative hydrogen evolution when fed with glutamate. Genome sequencing revealed 222 single nucleotide variances (SNVs) between the reference genome of C. sphaeroides strain 2.4.1 and the analyzed substrain H2. These affect 61 protein coding genes. A leucine-proline exchange is present in the σ⁵⁴ factor (rpoN2 gene), a global hydrogen and nitrogen metabolism regulator. We propose a model how this mutation alters DNA-binding properties that explain the unexpected organic nitrogen tolerance of hydrogen production. Transcriptomic analyses under varying glutamate concentrations support this finding. Thus, we present the first thorough genomic and transcriptomic analysis of a Cereibacter strain that shows promising metabolic characteristics for biotechnological hydrogen gas production from organic waste. These results suggest a potential target for strain optimization. Possibly, our key finding can be transferred to other hydrogen producing microorganisms.

New Geographical (Im)materialities in Rural Spaces for a Renewed Countryside in the Global North. Some Key Comments in the Rural Geography Debate

From the point of view of the new (im)materialities and the relevance of vernacular house in the process of rural change and restructuring, this contribution comments some possible innovative ways of research in rural studies. The objective of the study is to bring the attention about the relevance of vernacular houses in the process of global rural change and restructuring and their particular expressions in localities and vernacular houses. The methodology in qualitatively based on auto-biographical and ethnographical research based in three houses of study in a marginal rural area of central Spain. The main conclusions suggest a process of hybridization of people and vernacular houses with two different circuits: new comers and traditional populations.

A Review on Significant Role of Additive Manufacturing in Biomedical Applications

The rapid development of manufacturing sector has created a platform for implementing novel technologies such as additive manufacturing (AM). AM or 3D printing, has generated a lot of interests in biomedical applications during the last decade with a variety of novel printed polymeric materials. 3D printing fabricates 3D object with layer-by-layer processing through computer-controlled programming software. It has innumerable applications including electronics, aerospace engineering, automobile industry, architecture and medical sectors. One of the most demanding sectors of 3D printing is biomedical engineering applications such as medicines, drug delivery system, surgical instruments, orthopedics, scaffolds, implants etc. The clinical ramifications of AM-made healthcare goods are being catalyzed by recent developments in biomaterials. This review paper aims to explain the concept of 3D printing and its significance in developing polymeric materials for biomedical applications. An inclusive survey has been conducted on the various techniques involved in printing the biomedical devices. The proper selection of polymeric materials is important for biomedical applications, especially from 3D printing point of view and this vital parameter has been considered in this review paper. According to our findings, more breakthroughs in biomaterials, are required for the success and expansion of AM technology in the biomedical applications.

A Possible Reconstruction of Hominini Phylogeny

Purification the Solvent for the Production of High-Density Polyethylene

The adsorption purification of gasoline fraction with NaX zeolites as a solvent for the production of high-density polyethylene at a large pilot plant with a layer height of the adsorbent layer from 1 to 8 m is considered. Removal of impurities of aromatic and unsaturated hydrocarbons, organosulfur impurities and water ensured the production of high-quality polyethylene. The main characteristics of the adsorption process (the dynamic activity of zeolite NaX, the length of the mass transfer zone) in a wide range of flow rates of the cleaned raw materials are determined, allowing the calculation of the adsorber without applying the principles of large-scale transition. 

The Interplay between Experimental Data and Uncertainty Analysis in Quantifying CO₂ Trapping during Geological Carbon Storage

Numerical simulation is a widely used tool for studying CO₂ storage in porous media. It enables the representation of trapping mechanisms and CO₂ retention capacity. The complexity of the involved physicochemical phenomena necessitates multiphase flow, accurate fluid and rock property representation, and their interactions. These include CO₂ solubility, diffusion, relative permeabilities, capillary pressure hysteresis, and mineralization, all crucial in CO₂ trapping during carbon storage simulations. Experimental data is essential to ensure accurate quantification. However, due to the extensive data required, modeling under uncertainty is often needed to assess parameter impacts on CO₂ trapping and its interaction with geological properties like porosity and permeability. This work proposes a framework combining laboratory data and stochastic parameter distribution to map uncertainty in CO₂ retention over time. Published data representing solubility, residual trapping, and mineral trapping are used to calibrate prediction models. Geological property variations, like porosity and permeability, are coupled to quantify uncertainty. Results from a saline sandstone aquifer model demonstrate significant variation in CO₂ trapping, ranging from 17% (P10 estimate) to 56% (P90), emphasizing the importance of considering uncertainty in CO₂ storage projects. Quadratic response surfaces and Monte Carlo simulations accurately capture this uncertainty, resulting in calibrated models with an R-squared coefficient above 80%. In summary, this work provides a practical and comprehensive framework for studying CO₂ retention in porous media, addressing uncertainty through stochastic parameter distributions, and highlighting its importance in CO₂ storage projects. 

A Position-based Hybrid Routing Protocol for Clustered Flying Ad Hoc Networks

Unmanned aerial vehicles (UAVs) have been used to establish flying ad hoc networks (FANETs) to support wireless communication in various scenarios, from disaster situations to wireless coverage extensions. However, the operation of FANETs faces mobility, wireless network variations and topology challenges. Conventional mobile ad hoc network and vehicular ad hoc network routing concepts have rarely been applied to FANETs, and even then they have produced unsatisfactory performance due to additional challenges not found in such networks. For instance, position-based routing protocols have been applied in FANET, but have failed to achieve adequate performance in large networks. Clustering solutions have also been used in large networks, but with a significant overhead in keeping track of the complete topology. Hence, to solve this problem, we propose a hybrid position-based segment-by-segment routing mechanism for clustered FANETs. This approach facilitates traffic engineering across multiple wireless clusters by combining position-based inter-cluster routing with a rank-based intra-cluster routing approach capable of balancing traffic loads between alternative cluster heads. Simulation results show that our solution achieves, on average, a lower power consumption of 72.5 J, a higher throughput of 275 Mbps and a much lower routing overhead of 17.5% when compared to other state-of-the-art end-to-end routing approaches.

Plant Proteins Availability in Europe and Asia: A Causality Analysis of Climate, Demographics, and Economic Factors

The article examines the availability of plant-based proteins in Europe and Asia, considering the challenges posed by climate, demographics, and economics. The availability of these proteins is crucial given the growing impact of climate, economic, and social variables. Indeed, these factors play a decisive role in the production and accessibility of plant-based proteins across countries. The study employed a causality analysis method using regression models to determine the relative impact of these factors on protein availability. Two indicators were prioritized: total national production and the daily accessible quantity per person. This approach made it possible to construct hypothetical trajectories, showcasing the interrelations between the different variables. The results show that the availability of plant-based proteins varies across regions. Factors such as rising temperatures, increasing pollutants, and rising prices of plant proteins are particularly concerning. In this context, legumes appear as a promising alternative. They offer resilience against climatic variations while being an excellent protein source. The findings also encourage rethinking our consumption. Meat, with its significant ecological footprint, should see its consumption decrease in favor of plant-based proteins, ensuring a more sustainable diet. To facilitate this transition, the importance of appropriate public policies and incentives for producing and consuming plant proteins is emphasized.

Climate Change Adaptation Strategies for Grape Cultivation in Yamanashi Prefecture of Japan

Climate change impacts agricultural production, especially fruits. Amongst fruits, the grape is economically valuable and highly affected by climate change. Therefore, climate adaptation strategies are essential in overcoming the detrimental effects of climate change on grape cultivation. The study summarises adaptation strategies for grape cultivation in general and focuses on climate change. The Yamanashi prefecture in Japan is taken for the case study. Our findings indicate a decline in grape production in Japan and Yamanashi prefecture. This is attributed to the effects of climate change. Following this, various support measures (adaptative, mitigation, others) provided by the Yamanashi government towards grape cultivation are summarised and analyzed. The study concludes by offering recommendations by drawing lessons from the literature review on adaptation strategies for grape cultivation, focusing on overcoming climate change impact in the context of Yamanashi prefecture.