Great needs always motivate the birth and development of new disciplines and tools. Here we propose in vitro BioTransformation (ivBT) as a new biomanufacturing platform, between the two dominant platforms—microbial fermentation and enzymatic biocatalysis. ivBT mediated by in vitro synthetic enzymatic biosystems (ivSEBs) is an emerging biomanufacturing platform for the production of biocommodities (i.e., low-value and bulk biochemicals). ivSEB is the in vitro reconstruction of artificial (non-natural) enzymatic pathways with numerous natural enzymes, artificial enzymes, and/or (biomimetic or natural) coenzymes without living cell’s constraints, such as cell duplication, basic metabolisms, complicated regulation, bioenergetics, and so on. The two great needs (i.e., food security and the carbon-neutral renewable energy system) have motivated the birth and development of ivBT. Food security could be addressed by making artificial food from nonfood lignocellulose and artificial photosynthesis for starch synthesis from CO2. The carbon-neutral renewable energy system could be addressed by the construction of the electricity-hydrogen-carbohydrate cycle, where starch could be a high density of hydrogen carrier (up to 14.8% H2 wt/wt) and an electricity storage compound (greater than 3000 Wh/kg). Also, ivBT can make a number of biocommodities, such as inositol, healthy sweeteners (e.g., D-allulose, D-tagatose, D-mannose), advanced biofuels, polymer precursors, organic acids, and so on. The industrial biomanufacturing of the first several biocommodities (e.g., myo-inositol, D-tagatose, D-mannose, and cellulosic starch) would wipe off any prejudice and doubt on ivBT. Huge potential markets of biocommodities with more than tens of trillions of Chinese Yuan would motivate scientists and engineers to address the remaining technical challenges and develop new tools within the next decade.
Drastically reducing emissions is essential to achieve the Paris Agreement’s (PA) goal of keeping global temperature well below 2 °C, ideally at 1.5 °C. With regard to residual emissions, however, a demand for negative emission technologies (NETs), also known as carbon dioxide removal (CDR), remains. NETs are particularly necessary to reach net-zero goals by offsetting emissions in hard-to-abate sectors. This article examines the distinction between “engineered” and “nature-based” removals from the perspective of international climate change law. To that end, the relevant legal norms in the United Nations Framework Convention on Climate Change (UNFCCC), the Kyoto Protocol (KP), and the PA are interpreted—with a particular emphasis on two engineered removals: bioenergy with carbon capture and storage (BECCS) and direct air carbon capture and storage (DACCS). We posit that the three treaties establish a normative hierarchy that is more favorable towards so-called nature-based removals and less favorable to engineered removals (and even more favorable towards emission reductions).
This article commemorates the outstanding Russian scientists E.F. Gross and A.N. Terenin. It revisits their successors’ efforts to develop Terenin’s idea of using excitons, discovered by Gross, for photocatalytic redox reactions on wide-gap semiconductors. Terenin proposed ZnO as the subject of study. To explore the possibility of replacing photogenerated electrons and holes in a redox reaction by an exciton being a quasi-neutral particle, the test reaction of the photoactivated oxygen isotope exchange (POIE) was studied. It was found that many years of initial unsuccessful attempts were due to the fact that the exciton energy is spent on luminescence. In our experiments, the excitons decayed non-radiatively, and the long-lived electron-donor F-type and hole V-type active centers were formed by creating the 2D surface nanostructure ZnO/ZnO1−x/O−. These centers allowed to obtain the reaction efficiency 5–8 times higher than with the interband transitions. Thus, the developed 2D surface nanostructure ZnO/ZnO1−x/O− resolved the problem of using an exciton in photocatalysis and demonstrated the perspective of this nanostructure as an efficient photocatalyst.
Establishing microbial cell factories has become a sustainable and increasingly promising approach for the synthesis of valuable chemicals. However, introducing heterologous pathways into these cell factories can disrupt the endogenous cellular metabolism, leading to suboptimal production performance. To address this challenge, dynamic pathway regulation has been developed and proven effective in improving microbial biosynthesis. In this review, we summarized typical dynamic regulation strategies based on their control logic. The applicable scenarios for each control logic were highlighted and perspectives for future research direction in this area were discussed.
The “SDG Future Cities” established by the SDG (Sustainable Development Goals) Promotion Headquarters of Cabinet Office of Government of Japan, are being expanded to all municipalities in Japan through government support for the efforts of leading municipalities. Tosa town is a small town with less than 4000 people with high aging population and surrounded by mountains in Kochi Prefecture. The town was selected as one of the SDG Future City in 2020. In this paper, through a literature review, interviews with the Tosa Town Office, and a questionnaire survey and interview with residents of Tosa town, we considered what young people need to do to achieve SDG-11. Although Tosa Town has a variety of local government initiatives, the definition of a town where young people can continue to live, as revealed in the literature review, was found to be insufficient in terms of “economic resources,” “decision-making,” “data,” and “knowledge”. However, it was found that “community leaders,” “nature/topography,” and “community/people” are the most important factors for creating a town where people can continue to live. As for future issues, it is necessary to take measures for “decision-making,” “data,” and “knowledge,” as well as to solve issues specific to Tosa town.
Depletion of non-renewable resources and health hazards of petroleum-based polymers and plastics has enforced the development of eco-friendly materials. The use of conventional plastics has to be minimized and can be replaced with environmentally friendly and sustainable bio-based polymers or biopolymers due to extensive environmental impact. A major share of petroleum-based polymers is used for polymeric composites with research focus on green composites and biocomposites containing renewable/bioderived matrix polymer and fillers from naturally occurring fibers. Biocomposites hold great promise to replace petroleum-based polymer composites owing to their lower cost, non-toxicity, abundance of raw material, renewability, and high specific strength. All these merits of biocomposites have led to an increment in the development of new biocomposites with enhanced properties, wide applicability and ever demanding criteria. The recently published review studies detailed the raw materials used, fabrication techniques, characterization, and applications including biodegradation and rheological studies performed in recent years. This review covers all the important properties of biocomposites along with detailed description of synthesis, properties, characterizations and applicability of these green composites in several areas. The review also focuses on their raw materials, types, recent biocomposites, processing techniques, characterizations, applications, and current challenges with future aspects.
This study explores the transient characteristics of a drain water heat recovery (DWHR) device employed for heat recovery from warm grey water in buildings. Experimental measurements were conducted to investigate the response time of the DWHR device under various flow conditions. The thermal performance of the system was assessed using both transient and steady-state effectiveness analyses. The findings reveal that the response time is influenced by the water volume within the system, with an increase observed, and by the water flow rate, which leads to a decrease in response time. Additionally, a decrease in effectiveness is noted when hot water is used in short and frequent intervals. Furthermore, an economic analysis demonstrates that considering the transient behavior of the device results in a significant overall decrease of 37% in annual savings. Specifically, the usage of sinks exhibits a reduction in annual savings by 56%, while showers show a decrease of 13% in annual savings.
The earliest writing in China is the oracle bone inscriptions of the Shang Dynasty, which records early Chinese, also known as oracle bone Chinese, which are all monosyllabic-words (1300 BC). In the Bronze Inscriptions of the Western Zhou Dynasty and later handed down documents, doublets appear (beginning in 1046 BC). At present, the philological academy believes that the doublets recorded with two Chinese single-characters come from reduplication of two single-character symbols, but there is no complete argument and reliable evidence. This article, by using the opposite method of argument, reversely assumes that the single-characters (monosyllabic words) come from doublets and tries to demonstrate it. The article proves the truth of the origin of doublets based on the word distribution and semantic correspondence between doublets and single-characters in “the Book of Poetry”, that is, doublets are the source and single-characters are flows. Among them, 39.66% of the doublets have no corresponding single-characters, and they are the characters created to record doublets; 41.92% of the meanings of doublets have nothing to do with the meanings of single-characters, which proves that the doublets does not come from the combination of single-characters; 12.46% of the meanings of doublets are interpreted as the meanings of single-characters, which are the subjective errors of later generations of interpreters; the remaining 5.66% are only associated with proclitics and enclitics rather than single-characters. Finally, the article proposes that doublets originate from a unique mechanism of expressive morphology, which is a new type of etymological theory outside the morphological grammar system, and can create various polysyllabic ideophones, including the onomatopoeia or mimetic words. The article proves that a language begins with the creation of words. In the prehistoric period before the oracle bone inscriptions, Chinese ancestors had invented a large number of distinctive doublets (AA), couplets (AB) and other polysyllabic words (xA, or ABB, ABA’B), or ideophones. Due to the difficulty of writing, the doublets were hidden in spoken language for hundreds of years. It was not until the time of “Book of Poetry” and “Book of History” in the bronze inscriptions of the Western Zhou Dynasty that it entered the history and has continued to this day. Doublets are the earliest Chinese words and the beginning of Chinese civilization.