1
Department of Food and Nutrition, Japan Women´s University, 2-8-1 Mejirodai, Bunkyo-ku, Tokyo 112-8681, Japan
2
Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, 1-308 Suematsu, Nonoichi-shi, Ishikawa 921-8836, Japan
*
Authors to whom correspondence should be addressed.
Received: 12 Dec 2022
Accepted: 03 Feb 2023
Published: 07 Feb 2023
Abstract
Planococcus maritimus strain iso-3 was previously isolated from
intertidal sediment in the North Sea and was found to produce a highly modified
C
30-carotenoid, methyl-5-glucosyl-5,6-dihydro-4,4’-diapolycopenoate,
as the final product. In this study, we analyzed the function of the carotenoid
terminal oxidase
crtP (renamed
cruO) and aldehyde dehydrogenase
aldH genes in
P. maritimus strain iso-3 and
elucidated the carotenoid biosynthetic pathway for this strain at the gene
level. We produced four novel C
30-carotenoids with potent singlet
oxygen-quenching activities, 5-glucosyl-5,6-dihydro-4,4’-diapolycopen-4’-oic
acid and its three intermediates, which were obtained using
E. coli cells carrying the
cruO (and
aldH) gene(s) in addition to the known
P. maritimus carotenogenic genes.
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© 2023 by the authors; licensee SCIEPublish, SCISCAN co. Ltd. This article is an open access article distributed under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
