GEE25-01
Media Partnership
between Biobreeding and GEE25-01
Genome engineering: CRISPR/Cas in cells and mice (GEE25-01)
Course overview
This course will provide training in genome editing and cell engineering in mammalian cells and mouse embryos using the highly efficient CRISPR/Cas9 system. Participants will learn design of CRISPR targets using bioinformatics tools, generation of gene knock-outs/knock-ins, and target validation using the most current technologies. The course will be split into two groups (mammalian cells OR mice) based on participants’ expertise.
Please note that all participants are expected to engage in all sessions. Participants should plan for approximately 1 hour extra per day for self paced learning modules.
Audience
This course is aimed at researchers who are familiar with basic molecular and cell biology techniques and who want to learn how to create an engineered mammalian cell line or mouse model using the most recent and advanced CRISPR/Cas9 system. No previous experience in genome editing is required.
Modules/resources
Learning outcomes
After this course you should be able to:
between Biobreeding and GEE25-01
Genome engineering: CRISPR/Cas in cells and mice (GEE25-01)
Course overview
This course will provide training in genome editing and cell engineering in mammalian cells and mouse embryos using the highly efficient CRISPR/Cas9 system. Participants will learn design of CRISPR targets using bioinformatics tools, generation of gene knock-outs/knock-ins, and target validation using the most current technologies. The course will be split into two groups (mammalian cells OR mice) based on participants’ expertise.
Please note that all participants are expected to engage in all sessions. Participants should plan for approximately 1 hour extra per day for self paced learning modules.
Audience
This course is aimed at researchers who are familiar with basic molecular and cell biology techniques and who want to learn how to create an engineered mammalian cell line or mouse model using the most recent and advanced CRISPR/Cas9 system. No previous experience in genome editing is required.
Modules/resources
- How to create a CRISPR/Cas9 experiment using various design tools
- Transfection optimisation for efficient gene editing
- Generation of knock-out and knock-in cell lines and mouse embryos
- Validation of genome-edited cell lines and embryos
Learning outcomes
After this course you should be able to:
- Design a CRISPR based project using editing tools for your target gene of interest
- Choose the right format of gene editing tool and delivery strategy for your cell type or embryos
- Edit, screen and validate the engineered models, embryo manipulation