Found 4 results
Open Access
Article
14 August 2025Wood Chips Ameliorate the Negative Effects of LDPE Microplastic on Wheat Growth and Soil Microbial Community
As an emerging pollutant, microplastics (MPs) pose a potential risk to ecosystem health due to recycling technology’s limitations and long-term durability. Wood chip amendments can enhance the holding capacity of water and nutrients and improve the soil structure and quality of terrestrial ecosystems. However, the effects of wood chip amendments on MPs-contaminated soil-plant systems are still unclear. In this experiment, we employed a combination of field experiments, soil and plant measurements, molecular techniques (DNA extraction and sequencing), and advanced statistical analyses. A comprehensive assessment was conducted on the effects of Low-density polyethylene (LDPE) MPs on soil properties, wheat growth, and soil microbial communities, and the potential of wood chips as a mitigating agent was also evaluated. The results indicated that wood chips improved soil nutrient content, enhanced the activity of enzymes related to carbon, nitrogen, and phosphorus cycling, and promoted crop growth, all of which were negatively affected by LDPE. The effect of MPs on fungi was greater than that on bacterial communities, and adding wood chips could improve the α-diversity of fungal communities rather than bacteria. LDPE may increase the abundance of pathotrophic fungal groups, such as Stachybotrys and Alternaria. However, certain pathotrophic groups have been found to potentially facilitate the degradation of LDPE-MPs. LDPE reduced the symbiotroph groups and increased the competitiveness and complexity of the community in the microbial co-occurrence network. LDPE treatment inhibited the N-cycling bacteria group, while adding wood chips could promote most of the N-cycling bacteria groups. Wood chips increased saprotroph groups such as Trichoderma, which are able to degrade plastics. Wood chips emerge as a cost-effective and environmentally friendly solution to improve soil quality and mitigate the negative impacts of microplastics on crop growth.
Open Access
Article
15 April 2025Without Land and Water in Wallmapu (Ancestral Mapuche Territory): Exploring Causal Relationships and Perceptions in the Chol-Chol Watershed, La Araucanía, Chile
From a multi-variate database, causal relationships regarding water scarcity for human consumption in the Chol-Chol River basin were identified. The relationships were examined using the principal component analysis (PCA) statistical technique, and digital coverage was processed with ArcGIS 10.1, allowing for the construction of different thematic maps. Semi-structured interviews were conducted with various local actors, including Mapuche community leaders or lonkos (chiefs in the Mapudungun language) and local planners. The models with the greatest statistical significance are associated with the variables that measure land use changes between 2013 and 2017, particularly native forest and agricultural crops. In areas with greater changes in land use, there is less water availability and greater drinking water distribution by tanker trucks. A group of three models with the best goodness of fit (statistically significant) were identified. The models are related to the replacement of native forests with forest plantation (monoculture) and overexploitation of groundwater for irrigation. This model also links lower native vegetation cover in the southeastern part of the basin to agricultural uses on arable land, which is of higher quality than land in the north, and to lower drinking water consumption. The historical occupation processes of the Araucanía region (Wallmapu), the public policies of land and water (water emergency zone), climate change (decreases in flow and precipitation and increases in temperatures) are some of the driving forces behind land use change and water availability observed. An important innovation of this work has been the realization and discussion of the interviewees’ perceptions, showing different perspectives on a common problem; water scarcity. The interviews reveal diverse responses to the research question: What are the main variables related to the lack of water in Mapuche territory? The perception of Mapuche lonkos is that the lack of water is mainly associated with the rapid expansion of forest plantations. Local planners in the municipalities share a similar opinion.
Open Access
Perspective
18 February 2025The Reconfiguration of Social Bonds in the Digital Age: Virtual Connections vs. Face-to-Face Relationships
The article examines how smartphones and social media are transforming human interactions, challenging traditional concepts of friendship, intimacy, and belonging. Phenomena such as “phubbing” and constant connectivity are explored, highlighting the negative impacts of hyperconnectivity on the quality of face-to-face interactions and emotional well-being. While these technologies expand the reach of connections, they often lead to more superficial relationships, altering family, educational, and professional dynamics. Anthropological analysis is emphasized as essential for understanding these changes, revealing how digital practices vary across different cultural and social contexts. Ethnographic studies and innovative methodologies are suggested to investigate how digital technologies reshape identities, communities, and social hierarchies. The importance of an interdisciplinary approach, combining anthropology, psychology, and data science, is underscored to address the emerging challenges of the digital era and foster more authentic and healthy human relationships.
Open Access
Article
25 March 2024Proteomic Analysis of Pleurotus ostreatus Grown on Glucose and Xylose Mixtures in Submerged Fermentation Provides Insights into Differentiated Mycelial Composition
Pleurotus ostreatus, an edible white-rot fungus of great commercial and nutritional value, grows by metabolizing mainly glucose and xylose, the two major sugars in lignocellulosic biomass. In this study, a comparative proteomic analysis of P. ostreatus grown in submerged fermentation on a medium with glucose, xylose and mixtures of them as carbon sources was conducted. In the same conditions, the metabolic response of the fungus was evaluated in the production of the main nutritional components of the fungus such as proteins, lipids, and intracellular and extracellular polysaccharides. The proteomic analysis revealed that glucose and xylose upregulate different clusters of proteins. Glucose mainly up-regulates macromolecule metabolic processes, translation and glycolysis whereas xylose up-regulates, small molecule metabolic processes and tricarboxylic acid cycle (TCA). The mixtures show mostly similarities with the proteome response to glucose, although there are differential responses depending on xylose concentration. The carbon source type found to affect the basic macromolecule metabolic processes, with amino acids biosynthesis to differentiate mostly. An analysis of the upregulated proteins through the STRING database revealed that xylose upregulates mostly proteins related to amino acid biosynthesis. Leucine, Valine and Isoleucine biosynthesis pathways were found to be the most triggered pathway. All the branched-chain amino acids (BCAAs)-related enzymes intensities were gradually increased when xylose concentration was increased in the growth medium. BCAAs play an important role in the human diet so the enhancement of BCAAs biosynthesis pathway for P. ostreatus could convert it to a very remarkable protein substitute in human diet. These findings provide new insights into the proteomic and metabolic response of the fungus to the major sugars of lignocellulosic biomass, which are not well understood until now.
