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Development and Validation of a Novel 29-plex STR Multiplex System for Legal and Forensic Science

Perspectives in Legal and Forensic Sciences. 2024, 1(1), 10004; https://doi.org/10.35534/plfs.2024.10004
Chengliang yang 1    Hong Liu 2    Guanglin He 3,4    Xueyuan Liu    Zhonghao Yu 1    Changhui Liu 2    Ling Chen 1    Quyi Xu 2    Weian Du 1,6    Mengge Wang 2,3,4 *    Chao Liu 1,2,7 *   
1
Guangzhou Key Laboratory of Forensic Multi-Omics for Precision Identification, School of Forensic Medicine, Southern Medical University, Guangzhou 510515, China
2
Guangzhou Forensic Science Institute, Guangzhou 510030, China
3
Institute of Rare Diseases, West China Hospital of Sichuan University, Sichuan University, Chengdu 610041, China
4
Center for Archaeological Science, Sichuan University, Chengdu 610000, China
5
Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
6
AGCU ScienTech Incorporation, Wuxi 214174, China
7
Anti-Drug Technology Center of Guangdong Province, Guangzhou 510230, China
*
Authors to whom correspondence should be addressed.

Received: 01 Feb 2024    Accepted: 14 Mar 2024    Published: 20 Mar 2024   

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Abstract

Short tandem repeat (STR) analysis is the gold standard method in forensics for personal identification and paternity testing. Researchers have been working on developing novel multiplex systems containing more STRs for database construction and improvement of personal identification ability. This study's six-dye multiplex amplification system contained 29 autosomal loci, Y-indel, and Amelogenin. System optimization and performance measures were out according to the recommendations of the Scientific Working Group on DNA Analysis Methods, including PCR condition, sensitivity, mixture, inhibitor, species specificity, reproducibility, precision, stutter, concordance, and population study. The results showed that the complete profile was obtained with 125 pg of DNA input in a sensitivity study and a mixture ratio of 1:4. The full profile was observed with a hematin concentration ≤ 125 μmol/L, hemoglobin ≤ 200 μmol/L, EDTA ≤ 1.5 mmol/L, humic acid ≤ 1.5 μg/μL, indigo ≤ 12 mmol/L, and calcium ≤ 6.0 mmol/L. Meanwhile, the system also showed reasonable species specificity. Population genetic results showed the high performance of this panel with high informative and polymorphic loci, which possessed high estimates of the combined power of discrimination (1–7.16 × 10−35) and the combined power of exclusion (1–1.98 × 10−12) in southern Han Chinese populations.

Keywords

Forensic genetics; Short tandem repeat; Validation; 29-plex STR multiplex system

References

1.
Ensenberger MG, Lenz KA, Matthies LK, Hadinoto GM, Schienman JE, Przech AJ, et al. Developmental validation of the PowerPlex® Fusion 6C System. Forensic Sci. Int. Genet. 2016, 21, 134–144. [Google Scholar]
2.
Li S, Chen L, Wang Y, Xu Q, Liu H, Li Y, et al. Developmental validation of a 6-dye STR kit with 27 loci. Int. J. Legal. Med. 2018, 132, 335−342. [Google Scholar]
3.
Schmitt C, Benecke M. Five cases of forensic short tandem repeat DNA typing. Electrophoresis 1997, 18, 690–694. [Google Scholar]
4.
Edwards A, Civitello A, Hammond HA, Caskey CT. DNA typing and genetic mapping with trimeric and tetrameric tandem repeats. Am. J. Hum. Genet. 1991, 49, 746. [Google Scholar]
5.
Hagelberg E, Gray IC, Jeffreys AJ. Identification of the skeletal remains of a murder victim by DNA analysis. Nature 1991, 352, 427−429. [Google Scholar]
6.
Hares DR. Expanding the CODIS core loci in the United States. Forensic Sci. Int. Genet. 2012, 6, e52-4. [Google Scholar]
7.
Hares DR. Selection and implementation of expanded CODIS core loci in the United States. Forensic Sci. Int. Genet. 2015, 17, 33−34. [Google Scholar]
8.
Ensenberger MG, Hill CR, McLaren RS, Sprecher CJ, Storts DR. Developmental validation of the PowerPlex® 21 System. Forensic Sci. Int. Genet. 2014, 9, 169−178. [Google Scholar]
9.
Oostdik K, Lenz K, Nye J, Schelling K, Yet D, Bruski S, et al. Developmental validation of the PowerPlex® Fusion System for analysis of casework and reference samples: A 24-locus multiplex for new database standards. Forensic Sci. Int. Genet. 2014, 12, 69−76. [Google Scholar]
10.
Qu S, Li H, Li Y, Lv M, Yang F, Zhu J, et al. Developmental validation of the Microreader™ 20A ID system. Electrophoresis 2019, 40, 3099−3107. [Google Scholar]
11.
Jiang X, Guo F, Jia F, Jin P, Sun Z. Development of a 20-locus fluorescent multiplex system as a valuable tool for national DNA database. Forensic Sci. Int. Genet. 2013, 7, 279−289. [Google Scholar]
12.
Ge J, Sun H, Li H, Liu C, Yan J, Budowle B. Future directions of forensic DNA databases. Croatian Med. J. 2014, 55, 163−166. [Google Scholar]
13.
Wang Z, Zhou D, Jia Z, Li L, Wu W, Li C, et al. Developmental Validation of the Huaxia Platinum System and application in 3 main ethnic groups of China. Sci. Rep. 2016, 6, 31075. [Google Scholar]
14.
Green R, Elliott JL, Norona W, Go F, Nguyen VT, Ge J, et al. Developmental validation of VeriFiler™ Plus PCR Amplification Kit: A 6-dye multiplex assay designed for casework samples. Forensic Sci. Int. Genet. 2021, 53, 102494. [Google Scholar]
15.
Zhu BF, Zhang YD, Shen CM, Du WA, Liu WJ, Meng HT, et al. Developmental validation of the AGCU 21+ 1 STR kit: a novel multiplex assay for forensic application. Electrophoresis 2015, 36, 271−276. [Google Scholar]
16.
Ou X, Chen W, Chen H, Zhao F, Zheng J, Tong D, et al. Null alleles of the X and Y chromosomal amelogenin gene in a Chinese population. Int. J. Legal. Med. 2012, 126, 513–518. [Google Scholar]
17.
Colaco S, Modi D. Genetics of the human Y chromosome and its association with male infertility. Reprod. Biol. Endocrinol. 2018, 16, 14. [Google Scholar]
18.
Excoffier L, Lischer HE. Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol. Ecol. Resour. 2010, 10, 564–567. [Google Scholar]
19.
Gouy A, Zieger M. STRAF-A convenient online tool for STR data evaluation in forensic genetics. Forensic Sci. Int. Genet. 2017, 30, 148−151. [Google Scholar]
20.
Huckenbeck W, Scheffrahn W, Scheil HG, Enczmann J, Takenaka O. DNA PCR typing of non-human primates. Anthropologischer Anzeiger 2001, 59, 243–249. [Google Scholar]
21.
Schlotterer C, Tautz D. Slippage synthesis of simple sequence DNA. Nucleic Acids Res. 1992, 20, 211–215. [Google Scholar]
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© 2024 by the authors; licensee SCIEPublish, SCISCAN co. Ltd. This article is an open access article distributed under the CC BY license (https://creativecommons.org/licenses/by/4.0/).